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The Lhcb protein and xanthophyll composition of the light harvesting antenna controls the ΔpH‐dependency of non‐photochemical quenching in Arabidopsis thaliana
Author(s) -
Pérez-Bueno Maria L.,
Johnson Matthew P.,
Zia Ahmad,
Ruban Alexander V.,
Horton Peter
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.03.040
Subject(s) - xanthophyll , violaxanthin , non photochemical quenching , quenching (fluorescence) , chemistry , photoinhibition , thylakoid , biophysics , lutein , photosynthesis , photosystem ii , arabidopsis thaliana , chloroplast , zeaxanthin , photochemistry , biochemistry , mutant , biology , carotenoid , fluorescence , physics , quantum mechanics , gene
Nonphotochemical quenching (NPQ) is the photoprotective dissipation of energy in photosynthetic membranes. The hypothesis that the ΔpH‐dependent component of NPQ (qE) component of non‐photochemical quenching is controlled allosterically by the xanthophyll cycle has been tested using Arabidopsis mutants with different xanthophyll content and composition of Lhcb proteins. The titration curves of qE against ΔpH were different in chloroplasts containing zeaxanthin or violaxanthin, proving their roles as allosteric activator and inhibitor, respectively. The curves differed in mutants deficient in lutein and specific Lhcb proteins. The results show that qE is determined by xanthophyll occupancy and the structural interactions within the antenna that govern allostericity.