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Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue‐type mast cells
Author(s) -
Takano Hirotsugu,
Nakazawa Shunsuke,
Okuno Yasushi,
Shirata Naritoshi,
Tsuchiya Sohken,
Kainoh Takayuki,
Takamatsu Seigoh,
Furuta Kazuyuki,
Taketomi Yoshitaka,
Naito Yuko,
Takematsu Hiromu,
Kozutsumi Yasunori,
Tsujimoto Gozoh,
Murakami Makoto,
Kudo Ichiro,
Ichikawa Atsushi,
Nakayama Kazuhisa,
Sugimoto Yukihiko,
Tanaka Satoshi
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.03.033
Subject(s) - mast (botany) , connective tissue , microbiology and biotechnology , terminal (telecommunication) , process (computing) , chemistry , mast cell , biology , immunology , computer science , genetics , telecommunications , operating system
To understand physiological roles of tissue mast cells, we established a culture system where bone marrow‐derived immature mast cells differentiate into the connective tissue‐type mast cell (CTMC)‐like cells through modifying the previous co‐culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.