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Unconventional secretion of fibroblast growth factor 2 and galectin‐1 does not require shedding of plasma membrane‐derived vesicles
Author(s) -
Seelenmeyer Claudia,
Stegmayer Carolin,
Nickel Walter
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.03.024
Subject(s) - vesicle , secretion , microbiology and biotechnology , microvesicles , chemistry , extracellular , membrane , fibroblast growth factor , biology , biochemistry , microrna , receptor , gene
Various molecular mechanisms of unconventional secretion of fibroblast growth factor 2 and galectin‐1 have been proposed. A non‐vesicular pathway that is based on direct translocation across the plasma membrane has been described. In other studies, however, release into the extracellular space of cell‐derived vesicles was implicated in both FGF‐2 and Gal‐1 secretion. Such vesicles were proposed to originate either from plasma membrane shedding or by the release of exosomes. Employing an inhibitor of plasma membrane blebbing and based on a quantitative biochemical analysis of cell culture supernatants for vesicles potentially carrying FGF‐2 or Gal‐1, we demonstrate that both FGF‐2 and Gal‐1 are not exported by shedding of plasma membrane‐derived vesicles.