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Spectroelectrochemistry of P700 in native photosystem I particles and diethyl ether‐treated thylakoid membranes from spinach and Thermosynechococcus elongatus
Author(s) -
Zhang Yanrong,
Nakamura Akimasa,
Kuroiwa Yoshinori,
Kato Yuki,
Watanabe Tadashi
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.02.075
Subject(s) - p700 , spinach , thylakoid , photosystem i , spinacia , chemistry , diethyl ether , ether , photochemistry , membrane , photosystem ii , photosynthesis , chromatography , biochemistry , chloroplast , organic chemistry , gene
The redox potentials ( E ∘′ ) of P700 in intact and diethyl ether‐treated thylakoid membranes as well as native photosystem (PS) I particles from spinach and Thermosynechococcus elongatus have been measured by a spectroelectrochemistry with an error range of ±2–3 mV. Stepwise removal of antenna pigments by ether treatment caused distinct shifts of the E ∘′ value with increasing degree of water saturation in ether; negatively from +471 to +428 mV for spinach, but positively from +423 to +436 mV for T. elongatus . Such a contrasting behavior is discussed by invoking the mode of action of ether on the microenvironments around P700.