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RME‐8 regulates trafficking of the epidermal growth factor receptor
Author(s) -
Girard Martine,
McPherson Peter S.
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.02.042
Subject(s) - endosome , epidermal growth factor receptor , endocytosis , epidermal growth factor , microbiology and biotechnology , intracellular , receptor , erbb3 , cell surface receptor , chemistry , cancer research , biology , biochemistry
We recently identified receptor‐mediated endocytosis 8 (RME‐8), a DnaJ domain protein localized to endosomes. We now demonstrate that RME‐8 depletion leads to decreased levels of epidermal growth factor receptor (EGFR) without influencing receptors that primarily recycle to the plasma membrane. Decreases in EGFR are detected at both surface and intracellular pools and result from increased rates of EGFR degradation. Interestingly, RME‐8 depletion also decreases EGFR levels in breast cancer cell lines in which overexpression of the EGFR family member ErbB2 has been shown to protect EGFR from degradation. These data implicate RME‐8 in sorting decisions influencing EGFR at the level of endosomes and point to RME‐8 as a potential regulatory target in ErbB2‐positive breast cancers.