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The tobacco gene Ntcyc07 confers arsenite tolerance in Saccharomyces cerevisiae by reducing the steady state levels of intracellular arsenic
Author(s) -
Mok Young Geun,
Lee Byoung Doo,
Kim Young Jin,
Lee Chang Eun,
Kim Dong Gwan,
Lee Joohyun,
Shim Jaekyung,
Meng Yuling,
Rosen Barry P.,
Choi Jong Soon,
Shin Hyoung Sun,
Kim Seong-Ki,
Lee June Seung,
Hwang Seongbin
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.02.030
Subject(s) - arsenite , intracellular , saccharomyces cerevisiae , activator (genetics) , yeast , biology , gene , gene expression , microbiology and biotechnology , biochemistry , arsenic , chemistry , organic chemistry
We cloned a plant gene, Ntcyc07 , conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3 , but repressed that of As(III) uptake channel FPS1 . Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3 , but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(III) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1 .