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Silencing of the MT1‐MMP/ G6PT axis suppresses calcium mobilization by sphingosine‐1‐phosphate in glioblastoma cells
Author(s) -
Fortier Simon,
Labelle Dominique,
Sina Asmaa,
Moreau Robert,
Annabi Borhane
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.01.061
Subject(s) - chemistry , matrix metalloproteinase , gene silencing , mobilization , microbiology and biotechnology , sphingosine 1 phosphate , sphingosine , biochemistry , cancer research , biology , receptor , history , archaeology , gene
The contributions of membrane type‐1 matrix metalloproteinase (MT1‐MMP) and of the glucose‐6‐phosphate transporter (G6PT) in sphingosine‐1‐phosphate (S1P)‐mediated Ca 2+ mobilization were assessed in glioblastoma cells. We show that gene silencing of MT1‐MMP or G6PT decreased the extent of S1P‐induced Ca 2+ mobilization, chemotaxis, and extracellular signal‐related kinase phosphorylation. Chlorogenic acid and (−)‐epigallocatechin‐3‐gallate, two diet‐derived inhibitors of G6PT and of MT1‐MMP, respectively, reduced S1P‐mediated Ca 2+ mobilization. An intact MT1‐MMP/G6PT signaling axis is thus required for efficient Ca 2+ mobilization in response to bioactive lipids such as S1P. Targeted inhibition of either MT1‐MMP or G6PT may lead to reduced infiltrative and invasive properties of brain tumor cells.