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Biochemical characterization of a chromosomal toxin–antitoxin system in Mycobacterium tuberculosis
Author(s) -
Zhao Longxuan,
Zhang Junjie
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.01.045
Subject(s) - antitoxin , operon , open reading frame , mycobacterium tuberculosis , biology , toxin , gene , ribonuclease , microbiology and biotechnology , genetics , rna , tuberculosis , peptide sequence , escherichia coli , medicine , pathology
In the present paper, we report the biochemical characterization of a chromosomal toxin–antitoxin (TA) system in Mycobacterium tuberculosis , consisting of the Rv1991c gene and its upstream open reading frame (ORF) termed Rv1991a . Rv1991c was characterized as a toxin with ribonuclease activity and Rv1991a as the antitoxin against Rv1991c. Rv1991a interacted with Rv1991c to form a complex. A promoter located immediately upstream of Rv1991a was identified. Both Rv1991a and the Rv1991a–Rv1991c complex were able to bind to the promoter region of the Rv1991a–Rv1991c operon, indicating that the expression of the Rv1991a–Rv1991c operon can be autoregulated.