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Mitochondrial function, content and ROS production in rat skeletal muscle: Effect of high‐fat feeding
Author(s) -
Hoeks Joris,
Briedé Jacob J.,
de Vogel Johan,
Schaart Gert,
Nabben Miranda,
Moonen-Kornips Esther,
Hesselink Matthijs K.C.,
Schrauwen Patrick
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.01.013
Subject(s) - skeletal muscle , medicine , endocrinology , mitochondrion , respirometry , biology , chemistry , biochemistry
A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8‐week high‐fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respirometry, neither on a carbohydrate‐ nor on a lipid‐substrate. Interestingly, PPARGC1A protein increased by ∼2‐fold upon high‐fat feeding and we observed inconsistent results on different markers of mitochondrial density. Mitochondrial ROS production, assessed by electron spin resonance spectroscopy remained unaffected. Intramyocellular lipid levels increased significantly illustrating that a reduced innate mitochondrial function is not a prerequisite for intra‐muscular fat accumulation.