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Differential epigenetic regulation of Aiolos expression in human tumoral cell lines and primary cells
Author(s) -
Duhamel Marianne,
Navarro Pablo,
Cario-Andre Muriel,
Billot Katy,
Arrouss Issam,
Rebollo Angelita
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.01.010
Subject(s) - dna methylation , euchromatin , epigenetics of physical exercise , epigenetics , biology , histone methylation , epigenomics , microbiology and biotechnology , histone , ezh2 , cpg site , gene silencing , methylation , chromatin , jurkat cells , heterochromatin , genetics , gene expression , dna , gene , t cell , immune system
In order to investigate the epigenetic component of Aiolos regulation, we analyzed the methylation status of its 5′ CpG island in relation to histone modifications. Inhibition of CpG methylation restores Aiolos expression, as well as euchromatin‐associated markers, in U937 and 1106 mel cell lines. DNA methylation and low levels of euchromatin‐associated signatures are observed in U937 and 1106 mel cell lines, while the opposite characterizes Daudi, Jurkat, T and B cells. CpG methylation is not necessary to repress transcription in monocytes and melanocytes where silencing mechanism involves heterochromatin‐associated signature. We show that DNA methylation directs Aiolos silencing and chromatin status in tumor cell lines, while in primary cells is mainly regulated by histone modifications.