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Mycobacterial lipomannan induces MAP kinase phosphatase‐1 expression in macrophages
Author(s) -
Elass Elisabeth,
Coddeville Bernadette,
Kremer Laurent,
Mortuaire Marlène,
Mazurier Joël,
Guérardel Yann
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2008.01.007
Subject(s) - mapk/erk pathway , lipoarabinomannan , tlr2 , microbiology and biotechnology , phosphatase , p38 mitogen activated protein kinases , kinase , biology , mitogen activated protein kinase , mycobacterium bovis , chemistry , signal transduction , mycobacterium tuberculosis , phosphorylation , tlr4 , medicine , tuberculosis , pathology
Mycobacterial lipomannan (LM) and lipoarabinomannan (LAM) regulate macrophage activation by interacting with Toll‐like receptors (TLRs). The intracellular signalling pathways elicited by these complex molecules are poorly defined. We have demonstrated that LM purified from various mycobacterial species, but not LAM from Mycobacterium kansasii or Mycobacterium bovis BCG, induced expression of the MAP kinase phosphatase 1 (MKP‐1) in macrophages. Anti‐TLR2 antibodies, as well as specific ERK and p38 MAPK inhibitors, decreased MKP ‐ 1 transcription in LM‐stimulated cells. These findings suggest that the binding of LM to TLR2 triggers MAPK activation, followed by an up‐regulation of MKP ‐ 1 expression, which in turn may act as a negative regulator of MAPK activation.