Premium
Sigma factor SigC is required for heat acclimation of the cyanobacterium Synechocystis sp. strain PCC 6803
Author(s) -
Tuominen Ilona,
Pollari Maija,
von Wobeser Eneas Aguirre,
Tyystjärvi Esa,
Ibelings Bas W.,
Matthijs Hans C.P.,
Tyystjärvi Taina
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.12.030
Subject(s) - strain (injury) , sigma factor , synechocystis , acclimatization , gene , biology , microarray , cyanobacteria , gene expression , chemistry , microbiology and biotechnology , biochemistry , genetics , bacteria , promoter , anatomy
The role of the primary‐like sigma factor SigC was studied in Synechocystis . Under high temperature stress (48 °C) the ΔsigC inactivation strain showed a lower survival rate than the control strain. The ΔsigC strain grew poorly at 43 °C in liquid cultures under normal air. However, change to 3% CO 2 enhanced growth of ΔsigC at 43 °C. Differences in expression of many genes related to the carbon concentrating mechanisms between the control and the ΔsigC strain were recorded with a genome‐wide DNA microarray. We suggest that low solubility of CO 2 at high temperature is one of the factors contributing to the poor thermotolerance of the ΔsigC strain.