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Catalase T and Cu, Zn‐superoxide dismutase in the acetic acid‐induced programmed cell death in Saccharomyces cerevisiae
Author(s) -
Guaragnella Nicoletta,
Antonacci Lucia,
Giannattasio Sergio,
Marra Ersilia,
Passarella Salvatore
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.12.007
Subject(s) - catalase , superoxide dismutase , hydrogen peroxide , saccharomyces cerevisiae , yeast , acetic acid , enzyme , biochemistry , microbiology and biotechnology , chemistry , programmed cell death , dismutase , biology , apoptosis
To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA‐PCD), we compared Saccharomyces cerevisiae cells (C‐Y) and cells individually over‐expressing catalase T (CTT1‐Y) and Cu, Zn‐SOD (SOD1‐Y) with respect to cell survival, hydrogen peroxide (H 2 O 2 ) levels and enzyme activity as measured up to 200 min after AA treatment. AA‐PCD does not occur in CTT1‐Y, where H 2 O 2 levels were lower than in C‐Y and the over‐expressed catalase activity decreased with time. In SOD1‐Y, AA‐PCD was exacerbated; high H 2 O 2 levels were found, SOD activity increased early, remaining constant en route to AA‐PCD, but catalase activity was strongly reduced.