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The nucleoporin Nup358 associates with and regulates interphase microtubules
Author(s) -
Joseph Jomon,
Dasso Mary
Publication year - 2008
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.087
Subject(s) - nocodazole , microtubule , interphase , kinetochore , nucleoporin , microbiology and biotechnology , metaphase , astral microtubules , mitosis , biology , chemistry , cytoplasm , cell nucleus , genetics , cell , nuclear transport , cytoskeleton , chromosome , gene
The nucleoporin Nup358 resides on the cytoplasmic face of the interphase nuclear pore complex (NPC). During metaphase, its recruitment to kinetochores is important for correct microtubule‐kinetochore attachment. Here, we report that a fraction of endogenous Nup358 interacts with interphase microtubules through its N‐terminal region (BPN). Cells overexpressing the microtubule targeting domain of Nup358 displayed dramatic alteration in the microtubule organization including increased microtubule bundling and stability. Ectopic expression of BPN and full‐length Nup358 exhibited significantly higher levels of acetylated microtubules that were resistant to nocodazole, a microtubule depolymerizing agent. Furthermore, RNAi mediated depletion of Nup358 affected polarized stabilization of microtubules during directed cell migration, confirming the in vivo role of Nup358 in regulating interphase microtubules.