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Chemical DNA damage activates p21 WAF1/CIP1 ‐dependent intra‐S checkpoint
Author(s) -
Kan Qiuming,
Jinno Shigeki,
Yamamoto Hanako,
Okayama Hiroto
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.075
Subject(s) - g2 m dna damage checkpoint , chek1 , dna damage , cell cycle checkpoint , cdc25a , microbiology and biotechnology , cancer research , chemistry , biophysics , materials science , dna , biology , cell cycle , apoptosis , biochemistry
When cells progressing in G 1 phase are irradiated with UV light, two damage checkpoint pathways are activated: CHK1–Cdc25A and p53–p21 WAF1/CIP1 , both targeting Cdk2 but the latter inducing long lasting inactivation. In similarly irradiated S phase cells, however, p21 WAF1/CIP1 ‐dependent checkpoint is largely inactive. We report here that p21‐dependent checkpoint can effectively be activated and induce a prolonged S phase arrest with similarly extended inactivation of Cdk2 by association of p21 if mid‐S phase cells are damaged with a base‐modifying agent instead of UV light, indicating that the poor utilization of p21‐dependent checkpoint is not an innate property of S phase cells.