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Identification of a novel human MT5‐MMP transcript variant in multipotent NT2 cells
Author(s) -
Ross Heather H.,
Fillmore Helen L.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.074
Subject(s) - neurogenesis , biology , hippocampus , messenger rna , neuroscience , microbiology and biotechnology , gene , genetics
Membrane‐type 5 matrix metalloproteinase (MT5‐MMP) expression is ubiquitous in brain development while restricted to regions of neuroplasticity in adult. In the multipotent NT2 model of neurogenesis and differentiation, MT5‐MMP is differentially expressed with significantly higher mRNA levels in the differentiated neuronal hNT cells. MT5‐MMP cDNA cloned from NT2 cells unexpectedly revealed a novel sequence (MT5‐MMPvar) characterized by a 162 bp deletion. Both transcripts were identified in NT2, hNT and adult human hippocampus. In vitro, MT5‐MMPvar translated into a ∼55 kDa protein. It was also detected in NT2, hNT and adult human hippocampus. These results suggest more than one human MT5‐MMP transcript may exist in the central nervous system.