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Acute administration of 3,5‐diiodo‐ l ‐thyronine to hypothyroid rats affects bioenergetic parameters in rat skeletal muscle mitochondria
Author(s) -
Lombardi Assunta,
Lanni Antonia,
de Lange Pieter,
Silvestri Elena,
Grasso Paola,
Senese Rosalba,
Goglia Fernando,
Moreno Maria
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.073
Subject(s) - bioenergetics , thyronine , skeletal muscle , mitochondrion , medicine , endocrinology , triiodothyronine , oxidative phosphorylation , chemistry , respiration , cellular respiration , biology , thyroid , biochemistry , anatomy
We investigated the mechanism by which 3,5‐diiodo‐ l ‐thyronine (T2) affects skeletal muscle mitochondrial bioenergetic parameters following its acute administration to hypothyroid rats. One hour after injection, T2 increased both coupled and uncoupled respiration rates by +27% and +42%, respectively. Top‐down elasticity analysis revealed that these effects were the result of increases in the substrate oxidation and mitochondrial uncoupling. Discriminating between proton‐leak and redox‐slip processes, we identified an increased mitochondrial proton conductance as the “pathway” underlying the effect of T2 on mitochondrial uncoupling. As a whole, these results may provide a mechanism by which T2 rapidly affects energy metabolism in hypothyroid rats.