Premium
Direct neurite‐osteoblastic cell communication, as demonstrated by use of an in vitro co‐culture system
Author(s) -
Obata Koji,
Furuno Tadahide,
Nakanishi Mamoru,
Togari Akifumi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.065
Subject(s) - neurite , osteoblast , in vitro , microbiology and biotechnology , intracellular , chemistry , cell culture , prazosin , antagonist , receptor , biology , biochemistry , genetics
Using an in vitro co‐culture approach comprising cultured murine superior cervical ganglia and MC3T3‐E1 osteoblast‐like cells, we found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca 2+ mobilization and, after a lag period, osteoblastic Ca 2+ mobilization. SV did not have any direct effect on the osteoblastic cells in the absence of neurites. The addition of an α 1 ‐adrenergic receptor (AR) antagonist, prazosin, dose‐dependently prevented the osteoblastic activation that resulted as a consequence of neural activation by SV. These results demonstrate that osteoblastic activation occurred as a direct response to neuronal activation, which activation was mediated by α 1 ‐ARs in the osteoblastic cells.