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Effect of uncoupling protein‐1 expression on 3T3‐L1 adipocyte gene expression
Author(s) -
Senocak Fatih S.,
Si Yaguang,
Moya Colby,
Russell William K.,
Russell David H.,
Lee Kyongbum,
Jayaraman Arul
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.064
Subject(s) - thermogenin , uncoupling protein , oxidative phosphorylation , gene expression , uncoupling agents , chemistry , adipocyte , microbiology and biotechnology , adipose tissue , 3t3 l1 , brown adipose tissue , mitochondrion , biology , biochemistry , gene
The mitochondrial respiratory uncoupling protein 1 (UCP1) partially uncouples substrate oxidation and oxidative phosphorylation to promote the dissipation of cellular biochemical energy as heat in brown adipose tissue. We have recently shown that expression of UCP1 in 3T3‐L1 white adipocytes reduces the accumulation of triglycerides. Here, we investigated the molecular basis underlying UCP1 expression in 3T3‐L1 adipocytes. Gene expression data showed that forced UCP1 expression down‐regulated several energy metabolism pathways; but ATP levels were constant. A metabolic flux analysis model was used to reflect the gene expression changes onto metabolic processes and concordance was observed in the down‐regulation of energy consuming pathways. Our data suggest that adipocytes respond to long‐term mitochondrial uncoupling by minimizing ATP utilization.