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Poly(dG)–poly(dC) DNA appears shorter than poly(dA)–poly(dT) and possibly adopts an A‐related conformation on a mica surface under ambient conditions
Author(s) -
Borovok Natalia,
Molotsky Tatiana,
Ghabboun Jamal,
Cohen Hezy,
Porath Danny,
Kotlyar Alexander
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.058
Subject(s) - puc19 , mica , atomic force microscopy , dna , plasmid , molecule , morphology (biology) , chemistry , materials science , polymer chemistry , crystallography , nanotechnology , biology , biochemistry , organic chemistry , composite material , genetics
Three types of DNA: ∼2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)–poly(dC)], ∼2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)–poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)–poly(dC) is ∼30% shorter than that of poly(dA)–poly(dT) and the plasmid. This led us to suggest that individual poly(dG)–poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A‐form of DNA in contrast to poly(dA)–poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B‐form.