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A novel ferritin gene, SferH‐5 , reveals heterogeneity of the 26.5‐kDa subunit of soybean ( Glycine max ) seed ferritin
Author(s) -
Dong Xiangbai,
Sun Qiming,
Wei Dongping,
Li Jiahuang,
Li Jie,
Tang Bo,
Jia Qi,
Hu Wenjun,
Zhao Yongjuan,
Hua Zi-Chun
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.049
Subject(s) - ferritin , protein subunit , glycine , ceruloplasmin , complementary dna , biology , recombinant dna , biochemistry , gene , amino acid , microbiology and biotechnology
A novel ferritin cDNA, SferH‐5 , has been cloned from 7‐day‐old soybean seedlings. Putative SferH‐5 has 96% identity with SferH‐1 reported previously. All the five amino acid variants distributed in the mature region are not involved in highly conserved residues associated with ferroxidase activity center. We speculate that SferH‐5 encodes a novel 26.5‐kDa subunit of soybean seed ferritin, which is designated H‐5 in this study. Recombinant H‐5 was able to assemble, together with co‐expressed H‐2, as a functional soybean seed ferritin‐like complex, H‐5/H‐2. Our data reveal the potential heterogeneity of the 26.5‐kDa subunit of soybean seed ferritin.