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A soluble and active form of Wnt‐3a protein is involved in myogenic differentiation after cholesterol depletion
Author(s) -
Portilho Débora M.,
Martins Eliane R.,
Costa Manoel L.,
Mermelstein Cláudia S.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.047
Subject(s) - wnt signaling pathway , myogenesis , chemistry , beta (programming language) , microbiology and biotechnology , cholesterol , biochemistry , biology , signal transduction , in vitro , computer science , programming language
Cholesterol is one of the major lipids of plasma membranes. Recently, we have shown that cholesterol depletion by methyl‐β‐cyclodextrin (MβCD) induces the activation of the Wnt/β‐catenin pathway and enhances myogenic differentiation. Here, we show that MβCD‐conditioned media accelerates myogenesis in a similar way as MβCD does, suggesting that the effects induced by MβCD could be caused by soluble factors present in the culture medium. Soluble Wnt‐3 protein is significantly enhanced in MβCD‐conditioned medium. Wnt‐3a‐enriched media induces myogenesis as much as MβCD does, whereas Wnt‐5a‐enriched media inhibits. We suggest that Wnt‐3a is involved in the myogenic induction observed after cholesterol depletion.