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Arp3 is required during preimplantation development of the mouse embryo
Author(s) -
Vauti Franz,
Prochnow Blair Raymond,
Freese Elke,
Ramasamy Suresh Kumar,
Ruiz Patricia,
Arnold Hans-Henning
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.031
Subject(s) - trophoblast , blastocyst , biology , embryo , inner cell mass , phenotype , embryonic stem cell , genetics , microbiology and biotechnology , embryogenesis , andrology , gene , pregnancy , fetus , medicine , placenta
The role of Arp3 in mouse development was investigated utilizing a gene trap mutation in the Arp3 gene. Heterozygous Arp3 WT/GT mice are normal, however, homozygous Arp3 GT/GT embryos die at blastocyst stage. Earlier embryonic stages appear unaffected by the mutation, probably due to maternal Arp3 protein. Mutant blastocysts isolated at E3.5 fail to continue development in vitro, lack outgrowth of trophoblast‐like cells in culture and express reduced levels of the trophoblast marker Cdx2, while markers for inner cell mass continue to be present. The recessive embryonic lethal phenotype indicates that Arp3 plays a vital role for early mouse development, possibly when trophoblast cells become critical for implantation.