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Membrane topology of the Drosophila OR83b odorant receptor
Author(s) -
Lundin Carolina,
Käll Lukas,
Kreher Scott A.,
Kapp Katja,
Sonnhammer Erik L.,
Carlson John R.,
von Heijne Gunnar,
Nilsson IngMarie
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.11.007
Subject(s) - g protein coupled receptor , drosophila melanogaster , topology (electrical circuits) , transmembrane domain , membrane topology , drosophila (subgenus) , superfamily , biology , transmembrane protein , receptor , microbiology and biotechnology , genetics , gene , mathematics , combinatorics
By analogy to mammals, odorant receptors (ORs) in insects, such as Drosophila melanogaster , have long been thought to belong to the G‐protein coupled receptor (GPCR) superfamily. However, recent work has cast doubt on this assumption and has tentatively suggested an inverted topology compared to the canonical N out − C in 7 transmembrane (TM) GPCR topology, at least for some Drosophila ORs. Here, we report a detailed topology mapping of the Drosophila OR83b receptor using engineered glycosylation sites as topology markers. Our results are inconsistent with a classical GPCR topology and show that OR83b has an intracellular N‐terminus, an extracellular C‐terminus, and 7TM helices.