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CarO, an Acinetobacter baumannii outer membrane protein involved in carbapenem resistance, is essential for l ‐ornithine uptake
Author(s) -
Mussi María A.,
Relling Verónica M.,
Limansky Adriana S.,
Viale Alejandro M.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.063
Subject(s) - ornithine , acinetobacter baumannii , arginine , microbiology and biotechnology , histidine , lysine , imipenem , chemistry , bacterial outer membrane , amino acid , bacteria , biochemistry , biology , antibiotics , antibiotic resistance , escherichia coli , pseudomonas aeruginosa , gene , genetics
We previously associated the emergence of carbapenem resistance in Acinetobacter baumannii with the loss of an outer membrane (OM) protein designated CarO. CarO was found essential for l ‐ornithine uptake: CarO‐deficient strains were specifically impaired to grow only on l ‐ornithine, and failed to incorporate l ‐[ 14 C] ornithine from the medium. l ‐arginine, and histidine and lysine to a lower extent, could effectively compete for l ‐[ 14 C] ornithine uptake. l ‐ornithine also reduced A. baumannii sensitivity to imipenem, suggesting that both compounds compete for uptake. The overall results indicate that CarO participates in the selective uptake of l ‐ornithine, carbapenems, and other basic amino acids in A. baumannii .