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Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain
Author(s) -
Perier Aurélie,
Gourier Christine,
Pichard Sylvain,
Husson Julien,
Lajeunesse Evelyne,
Babon Aurélie,
Menez André,
Gillet Daniel
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.055
Subject(s) - diphtheria toxin , cell , intracellular , membrane , chemistry , cell membrane , adhesion , biophysics , cell adhesion , fusion protein , microbiology and biotechnology , ligand (biochemistry) , receptor , toxin , biochemistry , biology , recombinant dna , gene , organic chemistry
We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3‐ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell–cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell–cell adhesion by attaching protein ligands at the surface of cells.