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Histological mapping of biochemical changes in solid tumors by FT‐IR spectral imaging
Author(s) -
Petibois Cyril,
Drogat Benjamin,
Bikfalvi Andreas,
Déléris Gérard,
Moenner Michel
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.052
Subject(s) - pathology , spectral imaging , chemistry , biophysics , medicine , biology , geology , remote sensing
Fourier‐transform infrared (FT‐IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio‐allantoic membrane. In cell culture, glucose consumption and lactic acid release were higher in U87 cells. A549/8 cells were less sensitive to oxidative stress as observed through changes in fatty acyl chains. In vivo biochemical mapping of highly (U87) vs. poorly (A549/8) angiogenic tumors provided results comparable to culture models. Therefore, FT‐IR imaging allows detecting subtle chemical changes in tumors, which might be useful for diagnosis.