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Controllable self‐assembly from fibrinogen–gold (fibrinogen–Au) and thrombin–silver (thrombin–Ag) nanoparticle interaction
Author(s) -
Roy Shibsekhar,
Dasgupta Anjan Kumar
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.049
Subject(s) - thrombin , fibrinogen , silver nanoparticle , dynamic light scattering , transmission electron microscopy , conjugated system , coagulation , scanning electron microscope , nanoparticle , colloidal gold , microscopy , chemistry , fluorescence microscope , biophysics , flow cytometry , population , materials science , fluorescence , nanotechnology , platelet , biochemistry , organic chemistry , microbiology and biotechnology , biology , immunology , polymer , optics , pathology , medicine , psychiatry , environmental health , composite material , physics
Fibrinogen conjugated gold nanoparticles (fibrinogen–Au) and thrombin conjugated silver nanoparticles (thrombin–Ag) were synthesized by heating (90 °C) the proteins (50 μg protein/ml) with 1 mM AgNO 3 or AuCl 3 . The resultant particles were harvested and examined by flow cytometry, scanning electron microscopy (SEM), transmission emission microscopy (TEM), optical microscopy and dynamic light scattering. SEM and TEM images revealed that the fibrinogen–Au and thrombin–Ag particles interacted. The emergent bio‐nanoconjugate population could be controlled by addition of thrombin–Ag. The method may be exploited in parametrizing coagulation factors and other clinically important protein–protein interactions.