Function of a separate NH 3 ‐pore in Aquaporin TIP2;2 from wheat | Zendy

Bertl Adam | Zendy; Kaldenhoff Ralf | Zendy

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Function of a separate NH 3 ‐pore in Aquaporin TIP2;2 from wheat

Author(s) -

Bertl Adam,

Kaldenhoff Ralf

Publication year - 2007

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2007.10.034

Subject(s) - tetramer , ammonium , chemistry , ammonia , hydraulic conductivity , function (biology) , conductivity , ion , aquaporin , biophysics , inorganic chemistry , biochemistry , biology , organic chemistry , enzyme , genetics , ecology , soil water

Functional analysis of heterologously expressed TaTIP2;2 by means of stopped‐flow spectrometric studies provide evidence for water and ammonia conductivity. A series of experiments under increasing pH indicate that the gaseous NH 3 , rather than the ammonium ionNH 4 +was transported. Results from inhibitor studies strongly suggest that NH 3 is not transported in file with water, but through a separate pathway, which could be supplied by the 5th central pore in a tetramer conformation.

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