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Function of a separate NH 3 ‐pore in Aquaporin TIP2;2 from wheat
Author(s) -
Bertl Adam,
Kaldenhoff Ralf
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.034
Subject(s) - tetramer , ammonium , chemistry , ammonia , aquaporin , hydraulic conductivity , conductivity , inorganic chemistry , biophysics , biochemistry , organic chemistry , biology , soil water , enzyme , ecology
Functional analysis of heterologously expressed TaTIP2;2 by means of stopped‐flow spectrometric studies provide evidence for water and ammonia conductivity. A series of experiments under increasing pH indicate that the gaseous NH 3 , rather than the ammonium ionNH 4 +was transported. Results from inhibitor studies strongly suggest that NH 3 is not transported in file with water, but through a separate pathway, which could be supplied by the 5th central pore in a tetramer conformation.