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Vascular endothelial cell senescence mediated by integrin β4 in vitro
Author(s) -
Liu Xia,
Yin Deling,
Zhang Yun,
Zhao Jing,
Zhang Shangli,
Miao Junying
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.027
Subject(s) - senescence , integrin , reactive oxygen species , microbiology and biotechnology , small interfering rna , integrin alpha m , phospholipase , chemistry , endothelial stem cell , cell , in vitro , biology , biochemistry , rna , enzyme , gene
To understand whether integrin β4 is involved in vascular endothelial cell (VEC) senescence, we examined integrin β4 level changes, as well as P53 and reactive oxygen species (ROS) levels and alterations of phosphatidylcholine‐specific phospholipase C (PC‐PLC) activity before and after knocking‐down integrin β4 by small interfering RNA. We found integrin β4, P53 and ROS levels increased significantly, while Ca 2+ ‐independent PC‐PLC activity obviously decreased during VEC senescence. On the other hand, integrin β4 down‐regulation attenuated the senescence phenotype and reversed Ca 2+ ‐independent PC‐PLC activity, and P53 and ROS levels. The data suggested that integrin β4 might mediate VEC senescence through depressing Ca 2+ ‐independent PC‐PLC and elevating the levels of P53 and ROS.