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Ambient particulate matter induces alveolar epithelial cell cycle arrest: Role of G1 cyclins
Author(s) -
Zhang Jingmei,
Ghio Andrew J.,
Gao Mingxing,
Wei Ke,
Rosen Glenn D.,
Upadhyay Daya
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.020
Subject(s) - cyclin dependent kinase , microbiology and biotechnology , cyclin , cell cycle checkpoint , cell cycle , downregulation and upregulation , cyclin d1 , cyclin e , cyclin a , chemistry , g1 phase , cyclin b , biology , cell , biochemistry , gene
We hypothesized that the ambient air pollution particles (particulate matter; PM) induce cell cycle arrest in alveolar epithelial cells (AEC). Exposure of PM (25 μg/cm 2 ) to AEC induced cells cycle arrest in G1 phase, inhibited DNA synthesis, blocked cell proliferation and caused decrease in cyclin E, A, D1 and Cyclin E‐ cyclin‐dependent kinase (CDK)‐2 kinase activity after 4 h. PM induced upregulation of CDK inhibitor, p21 protein and p21 activity in AEC. SiRNAp21 blocked PM‐induced downregulation of cyclins and AEC G1 arrest. Accordingly, we provide the evidence that PM induces AEC G1 arrest by altered regulation of G1 cyclins and CDKs.