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Hypoxia/Notch signaling in primary culture of rat lymphatic endothelial cells
Author(s) -
Ota Hideki,
Katsube Ken-ichi,
Ogawa Jyun-ichi,
Yanagishita Masaki
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.009
Subject(s) - lymphatic system , lymphatic endothelium , microbiology and biotechnology , thoracic duct , hypoxia (environmental) , notch signaling pathway , transfection , in vitro , biology , endothelial stem cell , lymphatic vessel , cell culture , chemistry , signal transduction , immunology , oxygen , biochemistry , genetics , organic chemistry , cancer , metastasis
We have developed an improved intralumenal digestion method to get a long‐term primary culture of rat lymphatic endothelial cells (rLECs) that maintained their original phenotypes. rLECs in vitro under hypoxia retained their original lymphatic properties observed in the thoracic duct. Blocking Notch signal with a γ‐secretase inhibitor and transfection of a cDNA expressing a dominant negative form of Delta1 indicated that Notch signal downregulated VEGFR‐2 under hypoxia and inhibited cell migration. These findings indicated that Notch signal was still operative in mature lymphatic endothelial cells in response to the oxygen concentration.