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Cysteine cathepsins are not involved in Fas/CD95 signalling in primary skin fibroblasts
Author(s) -
Bojič Lea,
Petelin Ana,
Stoka Veronika,
Reinheckel Thomas,
Peters Christoph,
Turk Vito,
Turk Boris
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.005
Subject(s) - fas receptor , cathepsin , apoptosis , cathepsin b , chemistry , cycloheximide , cathepsin s , microbiology and biotechnology , cathepsin d , cysteine , cathepsin h , programmed cell death , biochemistry , biology , enzyme , protein biosynthesis
The potential role of cysteine cathepsins, especially cathepsin B, in Fas/CD95‐induced apoptosis was investigated using wild‐type and cathepsin B‐deficient primary skin fibroblasts. Apoptosis was induced with an anti‐Fas/CD95 antibody in the presence of cycloheximide and no difference was observed between the two genotypes. First cells with damaged mitochondria were observed ∼3 h post apoptosis induction and their number was significantly increased after 11 h. In contrast, cells with damaged lysosomes were only seen after 15 h with no difference between the two genotypes. Moreover, Bid cleavage was found to be diminished in cathepsin B‐deficient cells. These results suggest that cysteine cathepsins have no active role in Fas/CD95 apoptosis.