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Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi‐maternal embryos to term
Author(s) -
Kawahara Manabu,
Wu Qiong,
Ferguson-Smith Anne C.,
Kono Tomohiro
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.004
Subject(s) - genomic imprinting , germline , biology , embryo , oocyte , gene , genome , genetics , microbiology and biotechnology , chromosome , gene expression , andrology , dna methylation , medicine
Recently, we reported that the restored regulation of imprinted gene expression from two regions – H19 differentially methylated region ( H19 ‐DMR) and intergenic germline‐derived DMR (IG‐DMR) – is sufficient for accomplishing full‐term development in mice. In the present study, we determined the developmental ability of the bi‐maternal embryos (BMEs) containing the non‐growing oocyte genome with the IG‐DMR deletion (ng Δch12 ) and fully‐grown (fg) oocyte genome. Foetuses derived from ng Δch12 /fg BMEs were alive at E19.5 but could not survive further. Comparison with BMEs derived from Igf2 +/− ng/fg genomes suggests that bi‐allelic H19 expression might be involved in foetal development.