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Interaction of brain somatostatin receptors with the PDZ domains of PSD‐95
Author(s) -
Christenn Marcus,
Kindler Stefan,
Schulz Stefan,
Buck Friedrich,
Richter Dietmar,
Kreienkamp Hans-Jürgen
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.10.003
Subject(s) - pdz domain , somatostatin , somatostatin receptor , receptor , chemistry , neuroscience , microbiology and biotechnology , medicine , endocrinology , biophysics , biology , biochemistry
Using peptide affinity purification, we identified an interaction between somatostatin receptors SSTR4 and SSTR1 and PDZ domains 1 and 2 of the postsynaptic proteins postsynaptic density protein of 95 kDa (PSD‐95) and PSD‐93. The existence of the SSTR4/PSD‐95 complex was verified by coimmunoprecipitation from transfected cells and solubilized brain membranes. In neurons, dendritically localized SSTR4 partially colocalizes with postsynaptic PSD‐95. As functional parameters of the receptor, such as coupling to potassium channels, are not affected by the interaction with PSD‐95, the association may serve to localize the receptor to postsynaptic sites.