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Whole genome microarray analysis of C. elegans rrf‐3 and eri‐1 mutants
Author(s) -
Asikainen Suvi,
Storvik Markus,
Lakso Merja,
Wong Garry
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.09.043
Subject(s) - caenorhabditis elegans , mutant , gene , biology , genome , microarray analysis techniques , genetics , gene expression , microbiology and biotechnology
We performed genome wide gene expression analysis on L4 stage Caenorhabditis elegans rrf‐3 ( pk1426 ) and eri‐1 ( mg366 ) mutant strains to study the effects caused by loss of their encoded proteins, which are required for the accumulation of endogenous secondary siRNAs. Mutant rrf‐3 and eri‐1 strains exhibited 72 transcripts that were co‐over‐expressed and 4 transcripts co‐under‐expressed (>2‐fold, P < 0.05) compared to N2 wild type strain. Ontology analysis indicated these transcripts were over represented for protein phosphorylation and sperm function genes. These results provide additional support for the hypothesis that RRF‐3 and ERI‐1 act together in the endo ‐siRNA pathway, and furthermore suggests their involvement in additional biological processes.