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Production of the endocannabinoids anandamide and 2‐arachidonoylglycerol by endothelial progenitor cells
Author(s) -
Opitz Christiane A.,
Rimmerman Neta,
Zhang Yanmin,
Mead Laura E.,
Yoder Mervin C.,
Ingram David A.,
Walker J. Michael,
Rehman Jalees
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.09.032
Subject(s) - anandamide , 2 arachidonoylglycerol , endocannabinoid system , progenitor cell , chemistry , paracrine signalling , stimulation , microbiology and biotechnology , biology , endocrinology , cannabinoid receptor , biochemistry , stem cell , receptor , agonist
Recent studies have highlighted the importance of paracrine growth factors as mediators of pro‐angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid‐based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2‐arachidonoylglycerol by distinct human EPC sub‐types was measured using HPLC/tandem mass‐spectrometry. Anandamide release was highest by adult blood colony‐forming EPCs at baseline and they also demonstrated increased 2‐arachidonoylglycerol release with TNF‐α stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro‐inflammatory adhesion molecule CD106 (VCAM‐1) by TNF‐α.