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Molecular mechanism of molt‐inhibiting hormone (MIH) induced suppression of ecdysteroidogenesis in the Y‐organ of mud crab: Scylla serrata
Author(s) -
Imayavaramban L.,
Dhayaparan D.,
Devaraj Halagowder
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.09.029
Subject(s) - scylla serrata , moulting , hormone , mechanism (biology) , chemistry , microbiology and biotechnology , endocrinology , medicine , biology , anatomy , zoology , fishery , ecology , physics , larva , quantum mechanics
The present study was focused on the regulation of ecdysteroidogenesis in the Y‐organ of Scylla serrata during molting cycle. A strong expression of molt‐inhibiting hormone (MIH) and phosphorylation of ERK was predominantly observed in the postmolt and intermolt stages of Y‐organs, whereas protein kinase C, steroidogenic acute regulatory protein (StAR) and cytochrome P450 scc activity were exclusively seen in the premolt stages. Interestingly, inhibition of ERK phosphorylation by PD98059 in the early postmolt (A), middle postmolt (B) and intermolt (C) stages resulted in the prominent expression of PKC and StAR in the postmolt stages. This result indicates that phosphorylation of ERK is required for suppression of ecdysteroid biosynthesis with the involvement of protein kinase C, and StAR protein.