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The Ca 2+ ‐dependent exocytosis of enlargeosomes is greatly reinforced by genistein via a non‐tyrosine kinase‐dependent mechanism
Author(s) -
Prada Ilaria,
Cocucci Emanuele,
Racchetti Gabriella,
Meldolesi Jacopo
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.09.026
Subject(s) - exocytosis , genistein , tyrosine kinase , tyrosine , chemistry , tyrosine phosphorylation , receptor tyrosine kinase , microbiology and biotechnology , kinase , phosphorylation , biophysics , biochemistry , biology , signal transduction , membrane , endocrinology
Studies carried out by immunofluorescence, patch‐clamping and FM dye fluorescence consistently showed that the Ca 2+ ‐induced exocytosis of enlargeosomes, specific vesicles expressed by many cell types, is strongly reinforced by pre‐treatment of the cells with genistein, a wide spectrum blocker of tyrosine kinases, which also induces many additional effects. Various other blockers of tyrosine kinases, however, were ineffective, and the same occurred with drugs mimicking most of the rapid, non‐tyrosine kinase‐dependent effects of genistein. The reinforcement of enlargeosome‐regulated exocytosis, therefore, is a new effect of genistein and a peculiar property of the enlargeosome exocytosis, not shared by analogous processes.