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SVH‐B interacts directly with p53 and suppresses the transcriptional activity of p53
Author(s) -
Zhou Xinyuan,
Yang Guohua,
Huang Ruimin,
Chen Xiaotao,
Hu Gengxi
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.09.025
Subject(s) - transfection , apoptosis , microbiology and biotechnology , rna splicing , chemistry , rna interference , alternative splicing , mutant , biology , cancer research , rna , gene , messenger rna , biochemistry
We previously reported that inhibition of SVH‐B, a specific splicing variant of SVH, results in apoptotic cell death. In this study, we reveal that this apoptosis may be dependent on the presence of p53. Co‐immunoprecipitation and GST pull‐down assays have demonstrated that SVH‐B directly interacts with p53. In both BEL‐7404 cells and p53‐null Saos‐2 cells transfected with a temperature‐sensitive mutant of p53, V143A, ectopically expressed SVH‐B suppresses the transcriptional activity of p53, and suppression of SVH by RNA interference increases the transcriptional activity of p53. Our results suggested the function of SVH‐B in accelerating growth and inhibition of apoptosis is related to its inhibitory binding to p53.