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Two distinct human POM121 genes: Requirement for the formation of nuclear pore complexes
Author(s) -
Funakoshi Tomoko,
Maeshima Kazuhiro,
Yahata Kazuhide,
Sugano Sumio,
Imamoto Fumio,
Imamoto Naoko
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.09.021
Subject(s) - nuclear pore , gene , chemistry , nucleoporin , biophysics , computational biology , biochemistry , biology , nuclear transport , cell nucleus , cytoplasm
Pom121 is one of the integral membrane components of the nuclear pore complex (NPC) in vertebrate cells. Unlike rodent cells carrying a single POM121 gene, human cells possess multiple POM121 gene loci on chromosome 7q11.23, as a consequence of complex segmental‐duplications in this region during human evolution. In HeLa cells, two “full‐length” Pom121 are transcribed and translated by two distinct genetic loci. RNAi experiments showed that efficient depletion of both Pom121 proteins significantly reduces assembled NPCs on nuclear envelope. Pom121‐depletion also induced clustering of NPCs, indicating its role on maintenance of NPC structure/organization.