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The MlotiK1 channel transports ions along the canonical conduction pore
Author(s) -
Silverman William R.,
Heginbotham Lise
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.09.017
Subject(s) - thermal conduction , potassium channel , chemistry , biophysics , potassium , ion channel , ion , ammonium , biochemistry , materials science , biology , receptor , organic chemistry , composite material
Although the cyclic nucleotide‐modulated potassium channel from Mesorhizobium loti , MlotiK1, is easily studied using a 86 Rb + flux assay, its comparatively low activity raises serious concerns about the integrity of the purified protein. We investigated the pathway of uptake using a multi‐pronged approach. First, we probed the conduction pathway using quaternary ammonium compounds known to block conduction in eukaryotic K + channels. Second, we examined the effect of chemical modification of putative pore‐lining residues. Our results are consistent with ions traversing MlotiK1 along a conduction pathway like that of the eukaryotic channels, but at a much slower rate.