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Pseudovitamin B 12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions
Author(s) -
Santos Filipe,
Vera José L.,
Lamosa Pedro,
de Valdez Graciela F.,
de Vos Willem M.,
Santos Helena,
Sesma Fernando,
Hugenholtz Jeroen
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.09.012
Subject(s) - lactobacillus reuteri , corrinoid , chemistry , cobalamin , glycosidic bond , molecule , lactic acid , nuclear magnetic resonance spectroscopy , mass spectrometry , bacteria , ligand (biochemistry) , stereochemistry , biochemistry , lactobacillus , chromatography , vitamin b12 , organic chemistry , enzyme , biology , fermentation , genetics , methylation , methyltransferase , gene , receptor
We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitaminB 12activity. Here we report on the chemical characterisation of this corrinoid‐like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Co α ‐[ α ‐(7‐adenyl)]‐Co β ‐cyanocobamide or pseudovitaminB 12. This molecule differs from cobalamin in the α ‐ligand, where it has adenine instead of 5,6‐dimethylbenzimidazole bound in a α ‐glycosidic linkage to C‐1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin‐like molecule has been identified and the first microorganism reported to produce exclusively pseudo‐B 12.