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SNAP‐23 is not essential for constitutive exocytosis in HeLa cells
Author(s) -
Okayama Miki,
Arakawa Toshiya,
Mizoguchi Itaru,
Tajima Yoshifumi,
Takuma Taishin
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.046
Subject(s) - exocytosis , microbiology and biotechnology , secretion , mutant , snap , gene isoform , gene knockdown , hela , biology , cell culture , gene , biochemistry , genetics , computer graphics (images) , computer science
We applied the small interfering RNA (siRNA) technique and over‐expression of a dominant‐negative mutant to evaluate the role of SNAP‐23, a non‐neuronal isoform of SNAP‐25, in constitutive exocytosis from HeLa cells. Although the protein level of SNAP‐23 was reduced to less than 10% of the control value by siRNA directed against SNAP‐23, exocytosis of SEAP (secreted alkaline phosphatase) was normal. Double knockdown of SNAP‐23 and syntaxin‐4 also failed to inhibit the secretion. Furthermore, over‐expression of δC8‐SNAP‐23, a dominant‐negative mutant of SNAP‐23, did not abrogate SEAP secretion. These results suggest that SNAP‐23 is not essential for constitutive exocytosis of SEAP.