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Use of multicellular tumor spheroids to dissect endothelial cell–tumor cell interactions: A role for T‐cadherin in tumor angiogenesis
Author(s) -
Ghosh Sourabh,
Joshi Manjunath B.,
Ivanov Danila,
Feder-Mengus Chantal,
Spagnoli Giulio C.,
Martin Ivan,
Erne Paul,
Resink Therese J.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.038
Subject(s) - angiogenesis , downregulation and upregulation , cancer research , microbiology and biotechnology , endothelial stem cell , tumor microenvironment , biology , cell culture , chemistry , neovascularization , in vitro , tumor cells , gene , genetics
This study addresses establishment of an “in vitro” melanoma angiogenesis model using multicellular tumor spheroids (MCTS) of differentiated (HBL) or undifferentiated (NA8) melanoma cell lines. DNA microarray assay and qRT‐PCR indicated upregulation of pro‐angiogenic factors IL‐8, VEGF, Ephrin A1 and ANGPTL4 in NA8‐MCTSs (vs. monolayers) whereas these were absent in MCTS and monolayer cultures of HBL. Upon co‐culture with endothelial cell line HMEC‐1 NA8‐MCTS attract, whereas HBL‐MCTS repulse, HMEC‐1. Overexpression of T‐cadherin in HMEC‐1 leads to their increased invasion and network formation within NA8‐MCTS. Given an appropriate angiogenic tumor microenvironment, T‐cadherin upregulation on endothelial cells may potentiate intratumoral angiogenesis.