Premium
Fabrication of a dual substrate display to test roles of cell adhesion proteins in vesicle targeting to plasma membrane domains
Author(s) -
Hunt Stephen J.,
Nelson W. James
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.037
Subject(s) - vesicle , microbiology and biotechnology , adhesion , substrate (aquarium) , membrane , chemistry , cell adhesion , dual (grammatical number) , biophysics , cell , nanotechnology , biochemistry , materials science , biology , art , organic chemistry , ecology , literature
While much is known of the molecular machinery involved in protein sorting during exocytosis, less is known about the spatial regulation of exocytosis at the plasma membrane (PM). This study outlines a novel method, dual substrate display, used to formally test the hypothesis that E‐cadherin‐mediated adhesion directs basolateral vesicle exocytosis to specific sites at the PM. We show that vesicles containing the basolateral marker protein VSV‐G preferentially target to sites of adhesion to E‐cadherin rather than collagen VI or a control peptide. These results support the hypothesis that E‐cadherin adhesion initiates signaling at the PM resulting in targeted sites for exocytosis.