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Upstream stimulating factor‐1 mediates the E‐box‐dependent transcriptional repression of the plasminogen activator inhibitor‐1 gene in human mast cells
Author(s) -
Ma Zhongcai,
Jhun BongSook,
Oh Chad K.
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.034
Subject(s) - plasminogen activator inhibitor 1 , plasminogen activator , enhancer , negative regulatory element , transcription factor , activator (genetics) , effector , mast cell , microbiology and biotechnology , psychological repression , biology , gene , chemistry , regulatory sequence , gene expression , endocrinology , immunology , genetics
Plasminogen activator inhibitor (PAI)‐1 promotes development of asthma. PAI‐1 mRNA and protein are markedly induced in activated mast cells (MCs), a major effector cell type in asthma. However, regulatory mechanisms of PAI‐1 transcription in MCs are unknown. We present first evidence that PAI‐1 is transcriptionally regulated in human MCs (hMCs). In addition to three enhancer regions, we demonstrated that the E‐box at −566 bp to −561 bp is the negative regulatory element, and the specific and constitutive binding of the upstream stimulating factor‐1 to this E‐box is the key mechanism of the negative regulation of PAI‐1 expression in hMCs.