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Role of 3‐ O ‐sulfated heparan sulfate in virus‐induced polykaryocyte formation
Author(s) -
Tiwari Vaibhav,
ten Dam Gerdy B.,
Yue Beatrice Y.J.T.,
van Kuppevelt Toin H.,
Shukla Deepak
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.029
Subject(s) - heparan sulfate , colocalization , herpes simplex virus , sulfation , chemistry , in vivo , glycoprotein , microbiology and biotechnology , effector , virus , virology , biology , biochemistry , glycosaminoglycan , genetics
One way herpes simplex virus type‐1 (HSV‐1) spreads in vivo is by polykaryocytes formation. Here we demonstrate that polykaryocyte production during HSV‐1 spread in cultured human corneal fibroblasts (CF) required heparan sulfate (HS) and more specifically 3‐ O sulfated HS (3‐ O S HS). The polykaryocyte formation heavily depended on the expression of HS on target (CF) cells but not on glycoprotein expressing effector cells. Furthermore, we provide the first visual evidence of 3‐ O S HS and HSV‐1 gD colocalization during the membrane fusion process. Taken together our results provide novel insight into the significance of HS in polykaryocyte formation.