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The reactivation of DnaA(L366K) requires less acidic phospholipids supporting their role in the initiation of chromosome replication in Escherichia coli
Author(s) -
Aranovich Alexander,
Parola Abraham H.,
Fishov Itzhak
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.019
Subject(s) - dnaa , escherichia coli , mutant , microbiology and biotechnology , in vivo , biology , wild type , dna replication , in vitro , dnab helicase , origin of replication , biochemistry , chemistry , dna , genetics , gene , helicase , rna
DnaA(L366K), in concert with a wild‐type DnaA (wtDnaA) protein, restores the growth of Escherichia coli cells arrested in the absence of adequate levels of cellular acidic phospholipids. In vitro and in vivo studies showed that DnaA(L366K) alone does not induce the initiation of replication, and wtDnaA must also be present. Hitherto the different behavior of wt and mutant DnaA were not understood. We now demonstrate that this mutant may be activated at significantly lower concentrations of acidic phospholipids than the wild‐type protein, and this may explain the observed growth restoration in vivo.