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Absence of 4‐1BB increases cell influx into the peritoneal cavity in response to LPS stimulation by decreasing macrophage IL‐10 levels
Author(s) -
Shin Hyun-Hee,
Lee Ji-Eun,
Choi Hye-Seon
Publication year - 2007
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2007.08.015
Subject(s) - peritoneal cavity , lipopolysaccharide , stimulation , macrophage , chemistry , endocrinology , cell , medicine , microbiology and biotechnology , immunology , biology , biochemistry , in vitro , anatomy
Peritoneal injection of lipopolysaccharide (LPS) increased the influx of polymorphonuclear leukocytes and macrophages into the peritoneal cavity (PEC), with significantly higher cell numbers in the 4‐1BB‐deficient (KO) mice than in wild‐type (WT) mice. The peritoneal macrophages of KO mice contained less IL‐10 transcripts and protein than those of WT after LPS treatment, and immobilization of 4‐1BB‐Fc increased the level of IL‐10. Injection of IL‐10 resulted in lower cell numbers into the PEC of KO mice, suggesting that lower level of IL‐10 is responsible for stimulated cell influx in KO mice due to lack of 4‐1BB and 4‐1BBL interaction.